Buffer solution is usually a solution composed of "weak acid and its conjugate base" or "weak base and its conjugate acid" buffer pair, which can slow down pH changes when a certain amount of other substances are added. The function of Buffer is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested conforms to the range specified by the analytical method. In biochemical experiments and related research work, a large amount of buffer is often required to maintain the pH of the experimental system. Among them, TRIS and its derivative buffers are widely used in organic synthesis, coatings, fireproof materials, biochemical and pharmaceutical fields. So, what are the advantages and disadvantages in practical applications?
Get to know TRIS buffers first
Tris, also known as Tris, Tris buffer is more and more widely used, especially in nucleic acid and DNA related research experiments, it has more advantages than PBS phosphate buffer. On the other hand, Tris is also an important raw material for the synthesis of another biological buffer, TAPS. The advantages of Tris are very obvious. Its pH buffer range is 7-9, and its alkalinity is strong. It can only use this buffer to configure pH buffers ranging from acid to alkali; at the same time, because it does not precipitate with heavy metals, it can work together. , with little interference with biochemical processes. Therefore, Tris buffer is widely used not only for nucleic acid and protein
White matter solvent, there are many important uses. The low ionic strength of Tris buffers can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis.
Tris buffer also has some disadvantages to be aware of:
1. The pH of Tris buffer is greatly affected by the concentration;
2. It is greatly affected by temperature effects. For example, at room temperature of about 25 °C, Tris-buffer with pH of 7.8 is 8.4 at 4 °C and 7.4 at 37 °C. Therefore, the buffer prepared at 4 °C is taken to 37 °C When measured, its hydrogen ion concentration increased by a factor of 10. And it is below pH 7.5 and has poor buffering capacity.
Several common Tris-derived buffers
1. TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservatives, etc., PH7.4, is an isotonic buffered saline solution commonly used in biology, mainly used for immunohistochemistry and in situ hybridization, enzyme-linked immunosorbent assay In experiments such as washing of non-specifically bound antibodies in immunoblotting experiments, etc.
2. TBS is Tris-HCl buffered salt solution, which is isotonic salt solution plus Tris-HCl buffer, mainly composed of
Tris-HCl, NaCl, tween20, pH 7.2-7.5, mainly used for immunohistochemistry and in situ hybridization, enzyme-linked immunosorbent assay and other experiments, wash Western blot.
3. TE buffer is prepared from Tris and EDTA, a kind of electrophoresis buffer, PH8.0, mainly
It is used to dissolve nucleic acids and can store DNA and RNA stably.
4. TAE buffer is a buffer composed of Tris, acetic acid and EDTA, pH 7.2-8.4, which is a buffer system widely used for short-term electrophoresis of large fragments of DNA;
5. TBE buffer is a buffer solution composed of Tris base, boric acid and EDTA (ethylenediaminetetraacetic acid). TBE buffer is commonly used in agarose gel electrophoresis to analyze DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning.
Hubei Xindesheng is a high-tech company specializing in the research and development, production and sales of biological buffers, blood collection tube additives, chemiluminescent reagents and luminescent substrates. Fifteen years of rapid development, Desheng has a large R&D team and experimental base, and biological buffers have formed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. Now biological buffer products not only have a high market share in the domestic market, but also have been sold to dozens of countries around the world, and have established long-term and stable cooperation with many large-scale biomedical technology enterprises.