Model: | 1288 |
---|---|
Brand: | Sunlong |
Origin: | - |
Category: | Chemicals / Biochemical |
Label: | elisa kit , NF-Kb ELISA kit , human elisa kit |
Price: |
US $385
/ box
|
Min. Order: | 1 box |
Last Online:15 May, 2015 |
Materials supplied in the Test Kit
Materials provided with the kit |
96 determinations |
Storage |
|
1 |
User manual |
1 |
R.T. |
2 |
Closure plate membrane |
2 |
R.T. |
3 |
Sealed bags |
1 |
R.T. |
4 |
Microelisa stripplate |
1 |
2-8ºC |
5 |
Standard:18umol/L |
0.5ml×1 bottle |
2-8ºC |
6 |
Standard diluent |
1.5ml×1 bottle |
2-8ºC |
7 |
HRP-Conjugate reagent |
6ml×1 bottle |
2-8ºC |
8 |
Sample diluent |
6ml×1 bottle |
2-8ºC |
9 |
Chromogen Solution A |
6ml×1 bottle |
2-8ºC |
10 |
Chromogen Solution B |
6ml×1 bottle |
2-8ºC |
11 |
Stop Solution |
6ml×1 bottle |
2-8ºC |
12 |
wash solution |
20ml (30X)×1bottle |
2-8ºC |
Materials required but not supplied
1. 37 degrees incubator
2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips
4. Distilled water
5. Disposable tubes for sample dilution
6. Absorbent paper
Summary Procedure
Preparing reagents, samples and standards
Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at 37 ºC
Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at 37 ºC
Add stop solution
Measure the OD value within 10min
Package size:96T per box.
Validity&Storage:Six months(2-8 ºC )or Twelve months(-20 ºC )