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The working principle of chromatography freezer

Chromatography freezer (also called chromatography cabinet), and common storage cabinets have why to differ? It is how it works? And let Toffon confused for you a brief introduction of the working principle of chromatography freezer.

Insoluble covalently bound is through the biological molecules, there are many specific interaction between them to achieve specific and reversible binding, the binding force is called affinity. Affinity chromatography with the affinity of the two molecules in a fixed in the insoluble matrix, using molecular affinity between specific and reversible, the other a molecules were isolated and purified. Is fixed in the matrix molecules known as ligand, ligand and substrate is covalently bound to form affinity chromatography stationary phase, called affinity adsorbent. The affinity chromatography of first selection and separation of biological macromolecules have affinity substances as a ligand and the ligand in the appropriate Matrix. The preparation of affinity adsorbent column packing balance, when the sample solution by affinity column chromatography, biological molecules to be separated is with ligands occur specific binding, so stay in the stationary phase; and other impurities can not and ligand binding, still in the mobile phase, and with the elution liquid outflow, this column is only to be separated biomolecules. Through appropriate eluent from the ligand eluted, purified substances to be separated.

Two-phase flow distribution chromatography freezer is according to the work principle, the use of different physical and chemical properties of the difference and the establishment of chromatography technology for experiments provide suitable environmental temperature. All the chromatography system consists of two phases: a stationary phase, and the other one is the mobile phase. When to be separated, mixed with the mobile phase through a fixed phase, because each component physicochemical properties have differences, and two-phase interaction, adsorption, dissolution, combination of ability is different, in two phases (content), and with opposite before moving on to each group continually in redistribution. Segment collecting the effluent liquid, can be obtained in a sample containing the single A component, so as to achieve the purpose of separation of the components.