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CommaPrep™ plasmid midiprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 100 μg (NP100) or 200 μg (HP200).

Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.

Features

• Up to 100-200 μg high purity plasmid DNA
• Simple procedures and easy to operate
• Single protocol for high or low copy plasmids
• Suitable for various downstream applications

Typical results

Use NP100 to extract pET32a expression plasmid, and verify with double enzyme digestion.

Lane 1：Plasmid pET32a
Lanes 2-3：Plasmid by restriction digestion

Figure 1: Plasmid DNA analysed by restriction digestion

Experimental overview

Order information:

Note: It is recommended that the bacteria culture should be 30-50 mL and the elution volume should be 400-800 μL by using CommaPrepTM plasmid midiprep columns.