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The Canine Ehrlichia/Lyme/Anaplasma/Heartworm Combo Test based on sandwich lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into each sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Ehrlichia, Borrelia, Anaplasma recombinant antigens and heartworm antibodies respectively. If there are Ehrlichia, Borrelia, Anaplasma antibodies and Heartworm antigens in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Ehrlichia, Borrelia, Anaplasma antibodies and Heartworm antigens in the specimen.