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It can be used for both fluorescent RT-LAMP amplification and visual RT-LAMP amplification and detection, and is suitable for various rapid qualitative assays for nucleic acids. This product has the following features.
1. contains visible light and fluorescent dyes, can be used for both metal bath and water bath amplification with visible light to determine the results with the naked eye, and can also be used for real-time fluorescence detection with a fluorescent PCR instrument.
2. dUTP-UNG is included to prevent cross-contamination. 3.
3. higher specificity than RT-PCR, because RT-LAMP amplification uses 4-6 primers instead of two. 4.
4. sensitivity can be up to 10 copies/reaction (varies with primers), so the false negative rate is lower.
5. Can only be used for scientific research, only for qualitative testing, not for quantitative testing.