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Hanbon process chromatography columns can meet the use requirements of most chromatographic fillers. Its good filling effect and high column efficiency meet the needs of customers from pilot to large-scale production. Laboratory  LCC®chromatographic columns, MCC® manual adsorption chromatography column, and ACC® automatic axial compression chromatography columns are available.

Chromatography Columns in Commercialization Phase Selection

Along with the development of the biopharmaceutical industry, more and more companies are entering into clinical trials and commercial production, and a stable, reproducible and easy-to-use loading process is becoming a demand of more and more companies. Manual column loading faces many problems such as poor reproducibility, time-consuming method development, and complicated maintenance processes.

ACC automatic chromatography column was born, which controls the forward or reverse motion of the motor through position feedback or pressure feedback to realize the automatic lifting and lowering of the column head; through the valve opening and closing and pneumatic diaphragm pump to realize the filling and recovery of the gel. After years of precipitation, it has now developed into the latest generation of mature fully automatic intelligent chromatography columns.

Factors Affecting Column Efficiency

The theoretical plate number and symmetry factor of a chromatographic column are important indicators of column efficiency. Factors that affect the theoretical column plate number and symmetry factor of chromatography columns include:

column size and packing type

The nature of the mobile phase

the nature of the sample

Temperature and pH

In addition, the structural design of the chromatographic column itself also affects the column efficiency of the chromatographic column. Such as the material and surface roughness of the column wall, the tightness of the packing, the uniformity of the mobile phase distribution, etc. Excellent column performance is dependent on the structural design of the column.

We provide chromatography column qualification, small chromatography column, protein a chromatography column, mixed mode chromatography columns, low pressure chromatography columns, cation exchange column elution, etc. For more information, please feel free to contact us!