Model: | E0485h |
---|---|
Brand: | EIAab |
Origin: | Made In China |
Category: | Chemicals / Chemical Reagent |
Label: | ELISA Kit/EIAab , ELISA brand wuhan , NT-proBNP |
Price: |
US $596
/ box
|
Min. Order: | 1 box |
Last Online:18 May, 2022 |
Synonyms
NT-proBNP,; N-terminal prohormone of brain natriuretic peptide,;
Intended use
This immunoassay kit allows for thein vitro quantitative determination ofhuman N-terminal prohormone of brain natriuretic peptide,NT-proBNP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with anantibody specific to NT-proBNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific forNT-proBNPand Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NT-proBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NT-proBNPin the samples is then determined by comparing the O.D. of the samples to the standard curve.