Mouse Vasopressin ELISA试剂盒 uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse Vasopressin . During the reaction,Mouse Vasopressin  in the sample or standard competes with a fixed amount of Mouse Vasopressin  on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Mouse Vasopressin . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse Vasopressin  in the samples is then determined by comparing the O.D. of the samples to the standard curve.