Rat Ang1-7 ELISA試劑盒 uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat Ang1-7. During the reaction,Rat Ang1-7 in the sample or standard competes with a fixed amount of Rat Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Rat Ang1-7. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat Ang1-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.