Amersham Hybond-N+ 帶正電荷尼龍膜 0.45um 30cmx3m RPN303B
帶正電荷的尼龍膜與核酸高達600μg/cm2的結合能力。建議使用放射性和非放射性的化學發光法和chemifluorescence檢測系統。
Amersham公司Hybond™- N +產品在傳統的Southern雜交有着出色的靈敏度。核酸樣品可能是固定的，通過紫外線照射或烘烤，雖然紫外線固定是最好的最大的重複性。 Hybond N +是不適合碱印跡和固定。

Amersham Hybond™-N+

• Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm²¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
• Recommended for all high density membrane arrays.

 

Technical Information

Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.

 

• Multiple reprobing is possible without loss of membrane integrity.
   
• QC tested in genomic Southern blots using GE Healthcare products.
   
• Special sizes available on request.

 

Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C. bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.

 

Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.

 

TECHNICAL SPECIFICATIONS
Pore size	0.45 µm
Nucleic acid size required	> 50 bp