【Intended Use】
This kit is used to assay the CD5 antigen-like(CD5L)in the sample of Canine’s serum, blood plasma and other related biological liquid.
【Test principle】
　　　 This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Canine CD5 antigen-like (CD5L). Add CD5 antigen-like(CD5L)to the wells, which are pre-coated with CD5 antigen-like(CD5L)monoclonal antibody and then incubate. After that, add anti CD5L antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Canine CD5 antigen-like (CD5L) are positively correlated.