Model: | - |
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Brand: | Quicking |
Origin: | Made In China |
Category: | Industrial Supplies / Animal Husbandry Equipment |
Label: | total Aflatoxin , test , Aflatoxin kit |
Price: |
-
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Min. Order: | 1000 pc |
1. INTENDED USE
Quicking total Aflatoxin - Precise Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Aflatoxin residue in feed or grain sample.
Cut-off: 5 ppb base
Assay Time: 5 - 10 min
Sample: Feed or grain extraction
2. PRINCIPLE OF THE ASSAY
Quicking total Aflatoxin - Precise Rapid Test is based on competitive lateral flow immunochromatographic assay. The Aflatoxin conjugate in the test zone will capture the immuno-gold (colloid gold-AF antibody conjugate), when there is very little dissociative AF in the extraction. A visible red test band indicates a negative result when the control band (C zone) shows that the card is valid. The test band (T zone) will be not visible if AF is present in concentration of the cut-off value and above which explains a positive result.
3. KIT COMPONENT
- 10×foil pouches, each containing one TAF cassette with a pipette and a desiccant.
- 1×assay diluent (50 mL)
- 2×centrifugal tubes (15 mL)
- Products Manual
4. TEST PROCEDURE
- Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 2.0 g ground portion of the sample into the centrifugal tube (15 mL).
- Add 2 mL of water and 8 mL of ethyl acetate into the tube. Shake for 5 min. (If emulsification appears, do a centrifugation at 4000 rpm for 1 min.)
- Collect accurately 2 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by blowing wind.
- Redissolve the residues in the small beaker with an amount of the provided assay diluent. (REF: Table )
Cut-off (ppb) |
5 |
10 |
20 |
50 |
100 |
Assay Diluents (mL) |
0.4 |
0.8 |
1.6 |
4.0 |
8.0 |
- Take out the cassette from the foil pouch and place it horizontally.
- Suck the liquid and drip 3 drops into the assay sample hole “S”.
- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
5. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Aflatoxin is at or above 5 ppb in the samples.
Negative: The presence of both bands in C zone and T zone.
Invalid: No band appears in C zone.
6. STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
Quicking Biotech Co.,Ltd | |
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Country/Region: | Shang Hai - China |
Business Nature: | Manufacturer |
Phone: | 50947188 |
Contact: | Yohey Sun (International sales) |
Last Online: | 18 Feb, 2019 |