Principles

The Canine Adenovirus Ag Test Device is a immunochromatographic assay for the qualitative
detection  of  Canine  Adenovirus  antigen  (CAV  Ag)  in  canine  respiratory  tract  or  blood.  The
CAV  Ag Test Device has a letter of “T” and “C” as test line  and control line on the surface of
the card. Both the test line and control line in result window are not visible before applying any
samples. The control line is used for  procedural control. Control line should be always appear
if the test procedure is performed properly and the  test reagents of control line are working. A
purple  test  line  will  be  visible  in  the  result  window  if  there  are  enough  CAV  antigen  in  the
specimen. The specially selected  antibodies are used in test band as  both capture and detector
materials. These enable the device to identify CAV  antigen in canine respiratory  tract or blood
with a high degree of accuracy

Characteristics

1) One-step rapid test of canine adenovirus antigens
2) Rapid test results between 5 ~ 10 minutes
3) Expensive equipment not required
4) Easy storage and maintenance
5) High-purity and high-quality materials of the test kit increases its sensitivity and
specificity.

Materials (10 tests/kit)
1) Test -------------------------------------------------------------------------------------------- 10 units
2) Diluent (buffer) ------------------------------------------------------------------- 0.4   x 10 units
3) Disposable dropper -------------------------------------------------------------------------- 10 units
4) Swabs for sample collection --------------------------------------------------------------- 10 units

Composition

Specimen well (S : for dropping), Test line (T), and Control line (C) are marked on the device.
Inside  of  it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test
paper), and absorbent pad
Effect
Detection of canine adenovirus antigens from canine ocular or nasal discharge.

Uses

1) Specimen
Canine ocular discharge or nasal discharge.
2) Storage of specimen
a) Store specimen at 2~8°C for use within 24 hours or at -25°C for later use.
b) Keep the specimen at 22~25°C before use.
3) Test procedure
a)  Collect canine ocular or nasal discharge  with a swab and put the swab into a sample
tube containing 0.4    of buffer.
b) Swirl the buffer with the swab.
c) Drop 4 drops (100  ) into the specimen well.
d) Read the test results within 5 – 10 minutes.
♠ Invalid result after 10 minutes
4) Interpretation of the results
A  purple  band  should  appear  on  the  control  line  regardless  of  the  test  result.  The
presence of another band on the test line determines the result.
Control Line (C): The line should always appear regardless of the presence of the antigens
of  canine  adenovirus.  If  this  line  does  not  appear,  the  test  should  be
considered  invalid.  This  is  might  be  because  of  impure  buffer  or  the
lack of specimen. It should be tested again with another kit.
Test Line (T): The presence of antigens of canine adenovirus determines the test line.
1) Negative: Control line only appears.
2) Positive: Both Test and Control lines appear.
3) Retesting
a) Both Test and Control lines do not appear.
b) Test line only appears

5) Further examinations
This  test  is  for  primary  screening  only .  Consult  veterinarians  for  further  necessary
examinations to obtain clinical test results.

Precautions

1)    Use for canine in-vitro diagnostic purposes only.
2)    Use  within  10  minutes  after  opening  the  pouch  because  the  test  kit  is  very  sensitive  to
moisture and its effect may diminish.   
3)    Be careful of not touching the result window.
4)    Every specimen should be used with different dropper.
5)    For testing, buffer included only should be used.
6)    Do not use specimen being contaminated with microbes, which may cause false positive or
false negative result.
7)    Deal with specimen carefully. They can deliver unknown virus or infectious bacteria.
8)    Use disposable gloves when you suspect the infection caused by specimen. And wash your
hands later. 
9)    Dispose solid wastes after sterilizing them at 121 °C for over 1 hour.
10)  Do not the kit use when its pouch is torn, sealing is not  good shape and expiration date
passes.

Storage method

The  test  kits  store  at  2~30°C  can  be  used  for  24  months  after  manufacturing.  Do  not  keep
them in  a refrigerator. However, if they are stored under cold circumstances, keep them at room
temperature for 15 ~ 30 minutes before use.

Exchange

The  test  kits  are  manufactured  under  strict  quality  control  system.  Nevertheless,  if  they
deteriorate during delivery, ask our distributors for exchange.

Liability

The  entire  risk  due  to  the  performance  of  this  product  is  assumed  by  the  purchaser.  The
manufacturer  shall  not  be  liable  for  indirect,  special  or  consequential  damages  of  any  kind
resulting from use of this product.