Beating hit the carotid artery, internal jugular vein puncture, subclavian vein paracentesis, external carotid vein puncture; Preferable half lying position (analog in patients with severe) thoracentesis, pneumothorax extraction technique; Liver abscess puncture, can find liver area pressure point, have the breath training language tips, can puncture with breath rhythm; The injection technique, pericardiocentesis heart; Peritoneocentesis, preferable left and right side, line abdominal shifting dullness percussion training; Iliac crest bone marrow puncture; Beating hit a femoral artery and femoral artery puncture, femoral vein puncture; Before the aseptic technique training operation.