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INTENDED USE

Beta-Lactam Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Beta-Lactam residue in milk.

Cut-off:

Penicillin G      4 ppb,            Ampicillin    5 ppb,             Amoxicillin  5 ppb,
Oxacillin         15 ppb,           Cloxacillin 15 ppb,          Dicloxacillin   30 ppb,
Nafcillin          30 ppb,           Cefacetril  25 ppb,              Cefalexin   50 ppb,
Cefalonium    20 ppb,           Cefazolin   50 ppb,      Cefoperazone   50 ppb,
Cefquinome  15 ppb,            Ceftiofur    50 ppb,               Cefapirin  60 ppb.

Assay Time: 5-10 min
Sample: Milk

KIT COMPONENT
 
1. Foil pouches, each containing one strip, one pipette, one microwell and a desiccant
2. Product Manual

TEST PROCEDURE
 
1. Collect milk as testing sample.
2. Take out the microwells and strips of necessary number from the plastic canister. Recover the milk and test kit to room temperature (20-25 °C). Do not heat the milk.
3. Tear off the film of microwell.
4. Use pipette to transfer 200 μL of sample (a mark on the pipette) into the microwell.
5. Repeatedly suck and extrude the sample until all red reagents are completely dissolved.
6. Stand for 2 – 3 min and insert the unlabeled end of strip into the well.
7. Read the result in 5 minutes. Result after 10 minutes is considered invalid.

INTERPRETATION OF RESULTS
    
    

Positive:  Only clear C band appears.
Negative: The presence of both C band and T band.
Invalid:     No colored band appears in C zone regardless of whether T band appears.

SPECIFICITY

The results are negative when the rapid test is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Tetracyclines, Macrolides and Sulfonamides.

STORAGE

All materials must be stored at 2-8°C. The test kit is stable through the expiration date (12 months) marked on the foil pouch. Do not store the test kit in direct sunlight.

PRECAUTION

1. For best results, please strictly adhere to these instructions.
2. All reagents must be at room temperature before running the assay.
3. Do not remove rapid test from its pouch until immediately before use.
4. The milk sample should be fresh and homogeneous liquid without caking and precipitation.
5. Do not reuse the test strip.
6. Do not use the test beyond its expiration date marked on the foil pouch.
7. Do not use water, powdered milk as negative control. Use confirmed negative fresh milk as control.
8. The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

LIMITATION

Beta-Lactam Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. If you want a quantitative result, it is s ested to apply other method such as ELISA / HPLC in practice.