Anti-Bursal disease virus antibody ELISA kit

Infectious bursal disease virus antibody Rapid Assay

Catalog No.HC020-2

Summary

This kit use IBDV solid phase antigen to detect specific antibody against IBDV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times.

Reagents and contents

1 8× Coated microtiter strips: ready to use

2 1× Conjugate solution: ready to use

3 1× Washing solution: ready to use

4 1× Substrate A solution: ready to use

5 1× Substrate B solution: ready to use

6 1× Sample dilution: ready to use

7 1× Stopping solution: ready to use

Warning:Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor.

8 1× Positive control: ready to use

9 1× Negative control: ready to use

10 1× Instruction sheet

 

 

 

 

 

Test procedure

1 Sample preparation:

Dilutes the patient’s serum for the test with sample dilution at 1:100, e.g. 5 μl serum + 500μl sample dilution, and mix thoroughly.

2 Adding samples and controls

Leave well A1 for reagent blank. Pipette controls and samples as follows:

100μl of negative control and positive control respectively, and 100μl diluted samples each into remaining wells.

Incubation at 37℃for 30minutes. Discard off contents of the wells and add 1 drop washing solution to each well, fill all wells with distilled water completely, incubate for 30 seconds and discard off. Perform another 4 washing cycles as above. At the end of the washing step carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step.

3 Adding conjugate solution

Dispense 2 drops into all wells and incubate at 37℃ for 30 minutes. Discard the contents of the wells and wash 5 times as described in step 3.

4 Adding substrates

Dispense one drop of substrate A solution and B solution respectively, incubate at 37℃ for 10 minutes.

Dispense one drop of stopping solution into all wells. Zero the ELISA microtiter plate reader using the reagent blank well A1. Measure the absorbance at 450 nm.

Results

Judgment by microtiter plate reader

Cut-off value = 2.1×A- (absorbance of negative control)

Interpretation of results

A450 (patient) ≥ cut-off: positive

A450 (patient) ＜cut-off: negative

 

 

 

 

Notes

1 store at 2-8, make the temperature of the kit return to room temperature before use. screw down the cap after use, don't mix caps between diffirent bottles and don't mix components between diffirent kits

2 fill wells with distilled water when washing, 30 seconds each time, pour out the liquid. prohibit  to use tap water or any other water.

3 recommand to judge results with instruments to improve comparability of testing.