Estriol ELISA Test Kit

 

Principle

Estriol ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Estriol in feed,tissue and urine.The coupling antigens are coated on the micro-well stripes. The Estriolin the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Estriol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Estriol in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Estriol concentration in the sample.

 

Sample performance: detect Estriol residue in animal tissue and aquatic tissue(meat/liver)

 

Technical specification

Specification:96wells/kit

Sensitivity: 0.05 ppb     

Detect time: 45 min

 

Detection limit:

Tissue(fish, shrimp)………………………………………………0.1ppb

Pork/liver chicken/liver …………………………………………1ppb

Feed…………………………………………………………… 10ppb

Urine……………………………………………………………0.3ppb

 

Recovery rate 

Feed………………………………………………………………90±10%

Tissue……………………………………………………………85±10%

urine………………………………………………………………70±10%

 

Reaction rate

Estriol ……………………………………………………………100%

 

Storage: store at 2-8°C.

Shelf life: 12 months