Furazolidone (AOZ) ELISA test kit

 

Brief

Furazolidone is an antibacterial, may cause some serious side effects when taken with certain foods, beverages, or other medicines. Check with your health care professional for a list of products that should be avoided.

 

Principle

This assay kit is based on the competitive enzyme immunoassay for the detection of AOZ in the samples. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with AOZ concentration in the sample. This value is compared to the standard curve and the content of the corresponding AOZ is subsequently obtained.

 

Specifications

Sensitivity: 0.05 ppb

Specifications: 96 wells/kit

 

Detection limit:

Tissue (Chicken/duck/fish/shrimp/pork/beef/mutton)............................... 0.04 ppb

Liver (Chicken/duck/pork/beef/mutton)............................................ 0.04 ppb

Honey, milk, intestine, urine.................................................... 0.04 ppb

Shrimp, fish (some interference).................................................. 0.2 ppb

 

Recovery rate:

Tissue, liver.................................................................... 90±10%

Honey, milk, intestine, urine.................................................... 75±15%

 

Cross-reaction rate:

AOZ.................................................................................100% AHD................................................................................<0.1% AMOZ.............................................................................. <0.1%

SEM............................................................................... <0.1%
 

Storage: store at  2-8°C.not frozen

Shelf life : 12 months