Rotavirus is the primary causative agent of acute gastro-enteritis, especially in children less than 2 years old. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advance in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oral-faecal contact with an incubation period of 1-3 daysetc.
 
The Adenovirus is the second most common cause of viral gastro-enteritis in children (10-15%). This virus may also cause respiratory diseases and, depending on the serotype, also diarrhoea, conjunctivitis, cystitis,etc. At least 47 serotypes of adenovirus have been described, all sharing a common hexon antigen.Serotypes 40 and 41 are the ones associated with gastro-enteritis, whose main symptom is diarrhoea thatmay last between 9 and 12 days associated with temperature and vomits.
 
The test uses new homogenous immunochromatographic system with gold particules. It is a ready to use test which only needs a faecal sample dilution with the supplied ready to use dilution buffer. Specificity is ensured by using a monoclonal antibody conjugated with gold particules and directed against specific human genus-specific Rotavirus antigen or Adenovirus antigens. The immunochromatographic stick is coated with a polyclonal immunoreagent specific for genus-specific Adenovirus hexon antigens or rotaviurs VP6 antigen. Liquid sample and gold conjugate both migrate by capillarity and reach the first specific anti-Adenovirus polyclonal reagent. If Adenovirus is present in the sample, it is blocked and immunoreaction appears as a red-pink line. As sample still migrates, it reaches the second specific reagent line against Rotavirus,  then migrate to the non specific anti-mouse IgG which gives rise to a third red-pink line. This line indicates that the chromatography has been developed without hindrance. It appears also with negative samples.