One step HBsAg test is a rapid direct binding test for the visual detection of antigen in serum as an aid in the diagnosis of hepatitis b infection. Test result are read visually without any instrument.

One step HBsAg test is based on the principle of sandwich immunoassay for determination of HBsAg in serum. Monoclonal and polyclonal antibodies were employed to specifically identify HBsAg with high sensitivity. This one step test only takes about 10-20 minutes. The sensitivity of the test can reach to 5ng/ml.

Specimen collection

For serum, collect blood into container without anticoagulant. Allow the blood to clot and separate the serum from the clot. Use the serum for testing.

If the specimen cannot be tested on the day of collection, store the serum specimen in a refrigerator or freezer. Bring the specimens to normal room temperature before testing. Do not freeze or thaw the specimen repeatedly.

Nterpretation of result

Negative: only one pink colored band appears on the control region. No

            apparent band on the test region.

Positive:  in addition to a pink color band , a distinct pink colored

            band will also appear in the test region.

Invalid:   a total absence of color in both regions is an indication

             of procedure error and/or that test reagent

             deterioration has occurred.