Method：sandwich

Detection range：0.1mIU/L→40mIU/L

Sensitivity：0.05mIU/L

Assay length：1-5Hours

Detection Wavelengt：450nm

Research Area：Immunology

Price：inquiry

Quality guarantee period：for 12 months

Introduction

                                                                                                                                            

 

Reagents	Quantity	Reagents	Quantity
Coated ELISA plate	12-Well * 8 Tubes	Washing concentrate (30X)	20ml
Standard dilution	3ml	Instruction	1
Chromogen solution A	6ml	Seal plate membrane	2
Chromogen solution B	6ml	Hermetic bag	1
Streptavidin-HRP	6ml	Stop solution	6ml
Standard solution	0.5ml	Antibodies labeled with biotin	1ml

 

 

INTENDED USE

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma,tissue homogenates,cell lysates,cell culture supernates and other biological fluids.

 

STORAGE

For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃

 

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<8%  

Inter-Assay: CV<10%

 

ASSAY PROCEDURE SUMMARY 

1. Prepare all reagents, samples and standards; 

2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.

3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.

4. Add stop solution

5. Read the OD value within 10minutes. 

6. Calculate.