Introduction to Products

Product Name: Cooling agent ws-3

Chemical Name: N-Ethyl-2-(isopropyl)-5-methylcyclohexanecarboxamide

CAS No.: 39711-79-0

MF: C13H25NO

MW: 211.35

Appearance: White crystal powder

Odor: Mild cooling, slight menthol odor (almost odorless)

Detection Method: HPLC

Purity: ≥99%

Melting Point: 87ºC-102ºC

Flash Point: >100℃

Specific Rotation: -49° ~ -54° Water Solubility: Insoluble FEMA No.: 3455

EINECS No.: 254-599-0

Shelf Life: 2 years

Package: 1kg per bag with double plastic inside and aluminium foil bag outside, or 25kg per fibre drum with double plastic bag inside, or according to customers' requirement.

Storage: Store in cool and dry place. Keep away from strong light and heat.

Product Description:

Background:

WS 3 is an agonist of TRPM8 receptor with EC50 value of 3.7 μM [1].

Transient receptor potential cation channel subfamily M member 8 (TRPM8) is a Ca2+- and Na+-permeable ion channel and is activated by cooling agents and cold temperatures. TRPM8 belongs to TRP family and is expressed in sensory neurons.

WS 3 is an agonist of TRPM8 receptor and a cooling agent. In HEK293 cells expressed recombinant mouse TRPM8, WS 3 (30 μM) increased [Ca2+]i. WS 3 exhibited potency with EC50 value of 3.7 μM and induced 86% efficacy of the maximal response to icilin, the most potent agonist [1]. In HEK cells stably expressing either hTRPM8 or hTRPA1, WS 3 activated hTRPM8 and hTRPA1 with EC50 values of 2.2 and 120.6 μM, respectively. WS 3 was efficacious in eliciting cooling sensation [2].

References:

[1]. Behrendt HJ, Germann T, Gillen C, et al. Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol, 2004, 141(4): 737-745.

[2]. Klein AH, Iodi Carstens M, McCluskey TS, et al. Novel menthol-derived cooling compounds activate primary and second-order trigeminal sensory neurons and modulate lingual thermosensitivity. Chem Senses, 2011, 36(7): 649-658.

Advantages:

Food Grade Cooling Agent ws-3 For Cigarette/Filter Tip/Tobacco/Oral Product

 

1. There is a high cooling effect without other unpleasant feelings such as bitterness, burning, stinging or tingling sensations and can bring more stimulative, fresh and aromatic sensory to the entire mouth and throat.
2. Cooling agent ws-3 can solve the difficulty of developing new products with bad sensation.
3. When used with other flavors, it enhances the effects of the flavors. It also can be used with other cooling agents.
4. Cooling agent ws-3 is water-solubility is no more than 0.1%, it’s better than traditional cooling agents, so it can act on taste bud more wallop and has long cooling effect.

Functions:

1. Continuous and long lasting cooling and refreshing effect, having no hot, harsh and stinging sensation of Menthol and/or Peppermint.
2. Heat-Resistance: Heating under 200oC will not reduce the cooling effect, suitable to use on baking and other high temperature heating process.
3. Its cooling intensity can maintain 15-30 minutes, enriching the refreshing of product and having no burning pain, stabbing and numbness, it’s cooler than traditional Menthol products.
4. Low dosage: 30-100 mg/kg dosage will give good cooling effect.
5. Excellent compatibility with other flavors, it can enhance the effect of the flavors. It also can be combined using with other cooling agents.

Applications:

1. Daily use products: toothpaste, oral products, Air Freshener, skin cream, shaving cream, shampoo, sunscreen, shower cream.
2. Food products: confectionery products, chocolate, dairy produce, beer, distilled spirit, beverage, Chewing Gum.
3. Medicine: linctus, diminish inflammation ointment, dyspepsia, antipruritic, liniment, oral cavity acesodyne, heatstroke pill.
4. Others: cigarette, filter tip and other tobacco, it also can be used for driving insects.

Usage:

1. Soluble in Ethanol/PG firstly, then adding water solution.
2. Or dissolved in flavors firstly, then added into your products.
3. Mixed with dementholized peppermint oil can increase the fragrance signally.

Recommendation: 15-50mg/kg

Delivery Time: within 3 -7 days after receiving payments.

Shipping Details: DHL, TNT, FEDEX or EMS.

Payment Terms: T/T, West Union, Paypal and Escrow service.

Company Information:

Xi’an Taima Biological Co., LTD. was founded in 2008, is a technological export-oriented private enterprise with the Rights of Self-Managed Import and Export, specializing in active ingredients modernized and industrialization research, development, production, and sales of natural medical botany.

Our factory covers an area of 6,000 square meters equipped with advanced equipment and the production process is in accordance with FDA.

FAQ:

 

Q1: Could you provide free sample for test?

A1: We can provide you free sample for test, and you only need to pay the delivery cost.

Q2: Which payment terms do you accept?

A2: T/T, West Union, Paypal and Escrow service are available. But different payment terms against countries.

Q3: What's about the MOQ ?

A3: It depends on different products. Usually our MOQ is 1kg.

Q4: What’s the delivery lead time?

A4: We will make delivery within 3-7 days after receiving payment.

Q5: What about the delivery port?

A5: Main ports in China are available.

 

 

CERTIFICATE OF ANALYSIS

Product Name:	Cooling Agent WS-3	Manufacture Date:	May. 25, 2017
Batch Number:	TM170528	Certificate Date:	May. 28, 2017
Batch Quantity:	2000 kg	Expiration Date:	May. 24, 2019

 

 

Items	Specification	Result
Assay( HPLC)	≥99.0%	99.42%
Appearance	White crystal powder	Complies
Odor	Mild cooling, slight menthol odor (almost odorless)	Complies
Water Solubility	<0.1%	Complies
Melting Point	87ºC-102ºC	101.5ºC
Flash Point	>100ºC (Tag closed cup)	Complies
Thermo stability	Stable below 80ºC	Complies
Acidity (KOH)	≤1 mg/kg	0.15 mg/kg
Heavy Metals (as Pb)	≤10 mg/kg	Complies
Arsenic	≤3 mg/kg	Complies
Storage	Store in well-closed container at room temperature, protect from light, moisture and pest infestation.
Conclusion	Conform with specification.

Analyst: Xiao  you Corrector: Lin  zi Quality Assurance Officer: Zhang shiming