Hipure FFPE DNA Column

Model:	D51011
Brand:	Angen Biotech
Origin:	Made In China
Category:	Chemicals / Pharmaceutical Chemicals
Label:	-
Price:	US $180 / pc
Min. Order:	1000 pc

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Product Description

The HiPure FFPE DNA Column provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using Proteinase K, heat, and then purify the DNA with the FFPE DNA Column. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions withprotocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.

DNA Recovery	High quality total DNA (A260/A280 >1.8) can be eluted into small volumes (i.e., ≥25 µl) allowing for highly concentrated samples. The maximum DNA binding capacity of the provided spin column is ~25 µg
Processing Time	As little as 4 hours when processing large amounts of tissue. For maximum yields of the highest quality DNA, it is recommended to process samples overnight.
Equipment	Microcentrifuge, thermomixer or heat block/bath capable of 55°C and 90°C, isopropanol, beta-mercaptoethanol (optional).
Sample Size	Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm2 . It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens