1. Brief

The Beta-lactams, Tetracyclines, Streptomycin and Chloramphenicol Combo rapid test strip, based on Colloidal gold immunochromatography assay method, is used for testing Beta-lactams, Tetracyclines, Streptomycin and Chloramphenicol residues in milk and goat milk quickly.

2. Specifications

(1) 8 microwells and 8 dipsticks in one bottle      12 bottles / kit.

(2) Instruction                                        1 piece

3. Principle

The Beta-lactams, Tetracyclines, Streptomycin and Chloramphenicol Combo rapid test strip is based on competitive inhibition immuno-chromatographic principle. When the antibiotics residue in sample is higher than or equal to the detection limit, it will combine with labeled antibody, then inhibit the combination between colloidal gold antibody coated antigen on T line, making T line lighter than C line or T line invisible. Otherwise, T line is darker than C line or has same color with C line. No matter whether there is antibiotics residue in sample, C line is visible.

 4. Detection limit

Detection limit Tablet
Beta-lactams	Detection limit (ppb)	Beta-lactams	Detection limit (ppb)	Tetracyclines	Detection limit (ppb)	Streptomycin &Chloramphenicol	Detection limit (ppb)
Penicillin G	2~4	Cefquinome	8~15	Tetracycline	10~15	Chloramphenicol	0.3
Amoxicillin	2~3	Cefalotin	30~40	Oxytetracycline	5~10	Streptomycin	20~25
Ampicillin	2~3	Cefazolin	40~50	Chlortetracycline	5~10	Dihydrostreptomycin	15~20
Oxacillin	5~7	Cephalonium	8~10	Doxycycline	15~20
Cloxacillin	4~6	Cefacetrile	20~30
Piperacillin	6~8	Cefoperazone	3~5
Nafcillin	15~25	Cephapirin	8~10
Dicloxacillin	6~8	Ceftiofur	60~100

5. Operation procedures

1) Read the manual carefully before testing, return test strips and milk sample into room temperature before use.

2) Take bottles needed from the kit package, take out required microwells, making proper marks, Please use it in 1 hour. Seal the cap of the bottles, avoid moisture.

3) Use Micro-pipette to take 200ul of the samples into the microwells, then repeatedly absorb for 5 times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).

4) Incubate at (40±2℃) for 5min, then insert the strip into micro-well, put the end with MAX down into the liquid.

5) Insert the strip into the liquid for 5min, then read the result.

6. Test Result Interpretation

Color depth comparison of  T line and C line	Result judgment	Result analysis
T line > C line	Negative	antibiotics residue is lower than detection limit in sample
T line = C line	Weak positive	antibiotics residue is equal to detection limit in sample
T line < C line or T line is invisible	Positive	antibiotics residue is higher than detection limit in sample
C line is invisible	Invalid	The test dipstick is invalid, test again

7. Precautions

1) This product is suitable for pure milk of cattle and sheep and raw milk.

2) Test dipsticks and micro-wells are disposable,do not use test dipsticks out of date.

3) Please seal the bottle after taking out required test dipsticks. If can not use all 8 microwells at once, cup the rest and put it back to bottle and sealed.

4) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.

5) Store milk sample in cold, no more than 3 days.

6) Milk samples should be fully liquid without any agglomeration, sour and precipitation. Stir milk sample to even before test.

7) For milk sample with high fat content, chromatography is slow, recommending extension the second step for another 1 min.

8) Use the test dipsticks testing again for positive results.

9. Specificity

This product has no cross-reaction with Quinolones, Sulfonamides and Melamine etc.

10. Storage and expiry date

Storage:  Store at 2-8 ℃ in sealed, dry, and dark place, no frozen. 

Expiry date: 12 months; date of production is on box.