【Packaging specifications】 100tests/kit 100 person piece size：70*45*55mm 100 person piece weight: 0.026kg 【Detection Principle】 This kit is based on one-step RT-qPCR technique, virus ORF1ab and N gene are selected as amplification targets, and specific primers and fluorescent probes are designed for the detection of virus RNA in samples (ORF1ab gene probe labeled with FAM and N probe labeled with HEX). The kit also includes an endogenous internal standard detection system (the internal standard gene probe is labeled by Cy5), which is used to monitor the process of sample collection, nucleic acid extraction and PCR amplification, and can reduce the occurrence of false-negative results. The human RNase P gene is used as the internal standard. 【Expected Usage】 This product is a kit for rapid nucleic acid detection of virus based on fluorescent quantitative PCR probe method with specific primers and fluorescent probes designed according to ORF 1ab gene and N gene of virus. At the same time, UDG system can effectively prevent cross-contamination of PCR products.When used, only adding template, primer-probe mixture and water can carry out the test reaction, which greatly simplifies the operation process and reduces the error in the PCR operation process 【Test Method】 1. Reagent preparation (reagent preparation area) 2. Sample processing (sample processing area) 3. PCR amplification detection (nucleic acid amplification region) 4. Analysis of results