HEPES (4-Hydroxyethylpiperazineethanesulfonic acid) is a zwitterionic buffer, CAS No. 7365-45-9, this buffer is generally
It is often used in biochemical experiments to adjust the pH value of the reaction system, such as virus preservation solution or cell preservation solution.
have this ingredient. It has no toxic effect on cells, can control a constant pH range for a long time, not only can be used in the medium
As a buffer, it can also be used as a PH adjuster in cosmetics.
HEPES is a non-ionic amphoteric buffer with good buffering capacity in the pH range of 7.2-7.4. its larger
The characteristic is to maintain a relatively stable PH value during open culture or cell observation. Under these culture standards, cell culture flasks
The cap of the bottle should be tightened to prevent the small amount of carbonate often needed in the culture medium from escaping into the air.
How to use hepes buffer:
①Hepes buffer can be added to the prepared culture solution immediately according to the required concentration, and then filtered and sterilized. per 1000mlAdd 2.38 grams of HEPES to the culture solution, adjust the pH to 7.2 with 1N NaOH after dissolving, filter and sterilize before applying, at this time HEPES The application concentration is 10mmol/L.
②You can also prepare 100x stock solution (lmol/L), take 99ml culture medium and add 1ml stock solution before application, and finally
The use concentration is still 10mmol/L. lmol/L (100x) hepes buffer configuration: take 23.8g HEPES to dissolve 90ml
Double-distilled water, adjust the pH to 7.5-8.0 with 1N NaOH, then make up to 100ml with a titrant with water, filter sterilize, and store in bulk bottles
(2ml/bottle), store at 4°C or -20°C.
HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not degrade by autoclaving. According to relevant literature, such as
If an aqueous solution of HEPES is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H2O2) is produced so care should be taken:
1. The HEPES aqueous solution must be kept away from light so as not to affect the experimental results. After being configured as a solvent, it should be stored at 4°C. It is recommended that
Use it in the short term for better results!
2. When HEPES powder is placed, it is generally placed in a dry room and cannot be directly exposed to sunlight for a long time.
Desheng Biochemical is a professional manufacturer of biological buffers, with many types of products. Since itsestablishment in 2005, it hasWith more than ten years of R&D and production experience, the HEPES buffer produced by Desheng has high purity and wide application range. Welcome if neededGet in touch with us!