Cat. No. : Bio-PPK-250 (250μl/vial); Bio-PPK-500 (500μl/vial)

Concentration:Approximately 4.5mg/ml

 

Effective Size Range: 14~100KDa

 

 

 DESCRIPTION

Prestained protein Marker I is a mixture of purified proteins covalently coupled to a blue and red dye that resolves to 6 bands between 14 and 100kDa when electrophoresed. The product contains high-concentration of Prestained Protein Marker I, therefore little amount is necessary of them. It is designed for monitoring of protein separation during SDS-PAGE, verifying Western blot transfer efficiency, perfect for CBB, silver and negative staining. Color remains the same even after blotting. The Prestained Protein Marker I is supplied in gel loading buffer and is ready-to-use.

 

 

STORAGE

-     Store at -20℃, the shelf life is 1 year.

 

-     Aliquot and prevent freeze/thaw cycles.

 

 

CHARACTERISTICS

-     The separation state of sample can be presumed during electrophoresis.

-     Contain high-concentration of prestained proteins, therefore very little amount is necessary of them.

 

-     Very good lot-to-lot consistency.

 

-     Perfect for CBB, silver and negative staining.

 

-     Ready-to-use.

 

 

STORAGE BUFFER

2(W/V) % SDS       1mM EDTA-2Na

 

40mM DTT        70mM Tris-HCl (pH 6.8)

 

10(V/V) % Glycerol                 0.01% bromophenol blue

 

5% 2-mercaptoethanol          33mM NaCl

 

 GENERAL USE