Chloramphenicol Residue Rapid Test Device is used to qualitative detect Chloramphenicol in tissue samplesat the sensitivity of 0.1μg/kg（0.1ppb）. The whole time of assay is only approx. 30min.
SUMMARY
Chloramphenicol(CAP), one kind of broad-spectrum antibiotic, is effcetive to treat the infectious disease. It has been widely used in different lines of poltry, cattle, agriculture and beekeeping at present. But Chloramphenicol(CAP) can do harm to human body, it will inhibit the hematopoiesis function of marrow, thereby induce not only the Aplastic Anemia and agranulocytopenia, but also induce disease of human beings’ gastrointesinal tract and affect the nervous system. It has been probibited in most countries, inclduing China.
Radioimmunologic or gas chromatographic method is applied for Chloramphenicol residue. Howerver, enzyme immunoassays shows considerable advantages over conventional methods. Radioactive materials are no longer required, and the time is less than the gas chromatographic method.
The Chloramphenicol Residue Rapid Test Device is a rapid test to qualitatively detect the Chloramphenicol in specimens at the sensitivity of 0.1μg/kg（0.1ppb）. The major advantage of the one step test device is that the whole assay can be performed outdoors, and the results can be observed by eyes.
The principle of the test is the antigen-antibody reaction. The one step test device is a competitive immunoassay in which the detector reagent consists of colloidal gold particles coated with affinity purified anti-Chloramphenicol antibodies. The reagent in the assay is a Chloramphenicol-protein conjugate, which is immobilised by the membrane of the test device. Specimens migrate through the membrane. The more analyte in the sample the more effective it will compete with the Chloramphenicol immobilised in the membrane for binding to the limited amount of antibodies of the detector reagent.. If Chloramphenicol in the specimen is above 0.1μg/kg, it will thus prevent the binding of the detector reagent to the Chloramphenicol immobilised on the membrane, resulting in positive.