This immunoassay kit allows for the in vitro quantitative determination of Human Isoform 1 of Vitamin D-binding protein，DBP-I1, concentrations in serum, tissue homogenates and other biological fluids.

 

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBP-I1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DBP-I1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DBP-I1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DBP-I1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Reagent                                              Quantity

Assay plate                                                                     1

Standard                                             2

Sample Diluent                                                                    1 × 20ml

Assay Diluent A                                                                   1 × 10ml

Assay Diluent B                                                                   1 × 10ml

Detection Reagent A                                                           1 × 120μl

Detection Reagent B                                                           1 × 120μl

Wash Buffer(25 x concentrate)                         1 × 30ml

Substrate                                                                         1 × 10ml

Stop Solution                                                                             1 × 10ml

Plate sealer for 96 wells                                                      5

Instruction                                                                  1