Purpose

This kit allows for the determination of AMH concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human AMH level in the sample，use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add AMH to wells, Combined antigen which With HRP labeled，become antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMH in the samples is then determined by comparing the O.D. of the samples to the standard curve.