Purpose

This kit allows for the determination of BH4 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human BH4 level in the sample，use Purified Human BH4 antibody to coat microtiter plate wells, make solid-phase antibody, then add BH4 to wells, Combined BH4 antibody which With HRP labeled goat anti-Human become antibody-antigen- enzyme-antibody

complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BH4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.