Nitroimidazole ELISA Test Kit

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Model:	RN39SW
Brand:	reagen
Origin:	Made In United States
Category:	Chemicals / Chemical Reagent
Label:	-
Price:	-
Min. Order:	-

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Product Description

Product Description

REAGEN^™Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.

The unique features of the kit are:

High sensitivity.
A quick ELISA assay (less than 2 hours regardless of number of samples).

§ High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storageand Shelf Life

REAGEN^™Nitroimidazole ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Nitroimidazole -coated Microtiter Plate	1 x 96-well plate (8 wells x 12 strips)	2-8°C
Nitroimidazole Standards: Negative control 0.1ng/mL 0.3 ng/mL 0.9 ng/mL 2.7 ng/mL 8.1 ng/mL 100ng/mL (spiking, optional)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C
Antibody #1	7 mL	2-8°C *
HRP-Conjugated Antibody #2	12 mL	2-8°C *
TMB Substrate A	7mL	2-8°C
TMB Substrate B	7mL	2-8°C
20X Wash Solution **	40 mL	2-8°C
Stop Buffer **	7 mL	2-8°C
Duplicate dissolve solution	50mL	2-8°C

* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Honey	0.2

Member Information

reagen llc
Country/Region:	Guang Dong - China
Business Nature:	Manufacturer
Phone:	33118069
Contact:	chen (market)
Last Online:	29 Oct, 2012