Both anticoagulants and coagulants are essential additives for blood testing in vacuum blood collection tubes. Depending on the test items, different coagulants and anticoagulants will be added. Also as blood collection tube additives, what are the differences between the roles and uses of coagulants and anticoagulants? Who will be more useful?
First, the role and use of coagulants
When a coagulant is added to the vacuum blood collection tube, it can activate fibrin and turn soluble fibrin into insoluble fibrin aggregates, which can accelerate blood coagulation and shorten the test time. It is usually used together with serum separation gel, mainly used for Serum biochemistry (liver function, kidney function, myocardial enzyme, amylase, etc.), electrolytes (serum potassium, sodium, chloride, calcium, phosphorus, etc.), thyroid function, AIDS detection, tumor markers, serum immunity and other tests.
The role and use of anticoagulants
1. Heparin sodium and heparin lithium have the effect of strengthening the inactivation of serine proteins by antithrombin, thereby preventing the formation of thrombin and platelet aggregation, with minimal interference to blood components, without affecting the volume of red blood cells and causing hemolysis. Red blood cell osmotic fragility test blood gas, plasma osmotic volume, hematocrit, erythrocyte sedimentation rate and general biochemical determination. It is worth noting that excessive heparin can cause the aggregation of leukocytes, which cannot be used for leukocyte counting or classification of leukocytes.
2. Dipotassium, tripotassium, and disodium EDTA can effectively chelate calcium ions in blood, thereby preventing blood coagulation, having little effect on blood cell agglutination and the shape of blood cells, and can inhibit platelet aggregation , used for general blood tests. However, EDTA can inhibit or interfere with the polymerization of fibrin monomers during the formation of fibrin clots.
3. Trisodium citrate has an anticoagulant effect by acting on calcium ion chelation in blood samples, and can be used in most coagulation tests. And because trisodium citrate has little effect on MPV and other coagulation factors and is less toxic, it is often used for platelet function analysis and blood preservation. When the added trisodium citrate reaches a certain concentration, it can be used for erythrocyte sedimentation rate test.
According to the above analysis, it can be seen that coagulants and anticoagulants have their own uses and have irreplaceable roles in blood testing. As a high-quality manufacturer that can independently produce a full set of blood collection tube additives, Desheng can provide you with a one-stop service experience. If you have relevant needs, please click on the official website to inquire!