Porcine PGF2α ELISA試劑盒 was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Porcine PGF2α antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Porcine PGF2α antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine PGF2α amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Porcine PGF2αcan be calculated.