http://www.cd-genomics.com/cDNA-library-construction-service.html

CD Genomics has extensive experience in constructing high quality cDNA libraries in a vector of choice. The cDNA libraries are cloned into a standard high-copy vector, picked and arrayed into either 96-well or 384-well microtitre plates amenable to most high throughput DNA sequencing production systems. The libraries are well-suited for EST sequencing and other gene discovery applications. CD Genomics can also sequence ESTs from cDNA libraries via Sanger sequencing or 454 sequencing.

The steps of cDNA library construction

• Extraction of RNA from a variety of samples
• Generation of phage-or plasmid-based cDNA libraries from size-selected cDNA
• Directional cloning of cDNAs into any vector
• Production of a library with at least 106 cfu
• Library amplification to generate higher titer library
• Library delivered on plates, filters or glycerol stock
• Verification of library quality by randomly picking clones and determining insert sizes by restriction digest
• Verification of library complexity by DNA sequencing of the inserts from a subset of randomly picked clones (Optional)