Description
HiPure RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. 

Specific Activity: >2500 u/mg protein (>50 Kunitz units/mg protein)

Form: Contains 1gram HiPure RNase A, Lyophilized Powder, and 100ml Storage Buffer

Features

The HiPure RNase A is free of DNase activity. It is not necessary to heat it before use.

Applications

• Plasmid and genomic DNA preparation 
• Removal of RNA from recombinant protein preparations. 
• Ribonuclease protection assays
• Mapping single-base mutations in DNA or RNA 

 Storage

Store the HiPure RNase A powder at 4ºC or -20℃. Dissolved RNase A solution should be stored at -20ºC.