Synonyms

Thyroxine,;

Intended use

This immunoassay kit allows for the in vitro quantitative determination of generalThyroxineconcentrationsin serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toThyroxine, During the reaction,Thyroxinein the sample or standard competes with a fixed amount ofbiotin-labeledThyroxinefor sites on apre-coated Monoclonal antibodyspecific to Thyroxine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thyroxinein the samples is then determined by comparing the O.D. of the samples to the standard curve.