【Intended Use】
This kit is used to assay the Netrin-1(Ntn1)in the sample of Human ’s serum, blood plasma, and other related tissue Liquid.
【Test principle】
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Netrin-1(Ntn1)in samples. Add Netrin-1(Ntn1) to monoclonal antibody Enzyme well which is pre-coated with Human Netrin-1(Ntn1)monoclonal antibody, incubation; then, add Netrin-1(Ntn1)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Human Substance Netrin-1(Ntn1)of sample were positively correlated.