【Intended Use】
This kit is used to assay the Thyroxine(T4)in the sample of Chicken’s serum, blood plasma and other related biological liquid.
【Test principle】
　　　 This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Chicken Thyroxine (T4). Add Thyroxine(T4)to the wells, which are pre-coated with Thyroxine(T4)monoclonal antibody and then incubate. After that, add anti T4 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Chicken Thyroxine (T4) are positively correlated.