Heparinase I cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1). It is mainly used for quality control of heparin products.

General Information

Synonyms： Heparinase; heparin lyase; heparin eliminase

Gene Source: Native flavobacterium heparinum

EC Number: 4.2.2.7

CAS Number: 9025-39-2

MDL Number: MFCD00131317

Catalyzed Reaction: The enzyme cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.

Substrate Specificity: Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate).

Properties

Molecular weight: 42.8 kDa

 

Isoelectric point: 9.3 – 9.5

 

pH optimum for activity: 7.4

 

pH range for activity: 4 – 9

 

Optimal temperature range: 20 ℃ – 37 ℃

 

Purity: ≥ 98 % by reversed phase HPLC analysis.

 

Specific Activity: 100 IU/mg,1 IU/100 ul. One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 m mole unsaturated oligosaccharides from porcine mucosal heparin per minute at 37 ℃ and pH 7.0.

 

Stability: expiration is 12 months from manufacturing date, frozen at -20 ℃ in PBS.

 

Applications

• For the neutralization of heparin in blood and plasma samples before analysis.
• For similar in vitro neutralization of low molecular weight heparins.
• As integral part of in vitro diagnostic tests for the neutralization of heparin (blood clotting tests, platelet tests).
• In blood collection tubes for the neutralization of heparin.
• For the preparation of low molecular weight heparins from unfractionated heparin.
• As a research reagent (glycosaminoglycan degradation)
• For the preparation of disaccharides of heparin and the preparation of oligosaccharide libraries.

References

• Linker, A., and Hovingh, P. Meth. Enzymol. 28, 902, (1972)
• Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992)

Order Information

Catalog number: AG00-2519

Packing size: 1 IU

Price: 1300USD