Heparinase III cleaves heparan sulfate exclusively, and does not cleave unfractionated heparin or low molecular weight heparins. It is mainly used for quality control of heparin products.

General Information

Synonyms： Heparin sulfate eliminase; heparitinase I

Gene Source: Native flavobacterium heparinum

EC Number: 4.2.2.8

CAS Number: 37290-86-1

MDL Number: MFCD00131319

Catalyzed Reaction: The enzyme cleaves selectively, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active only towards heparan sulfate and does not cleave heparin or low molecular weight heparins.

Substrate Specificity: heparan sulfate.

Properties

Molecular weight: 70.8 kDa

Isoelectric point: 9.6 – 9.9

pH optimum for activity: 7.3

pH range for activity: 4 – 9

Optimal temperature range: 20 ℃ – 37 ℃

Purity: ≥ 98 % by reversed phase HPLC analysis.

Specific Activity: 100 IU/mg, 1 IU/200 μL. One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from heparan sulfate per minute at 30 ℃ and pH 7.5

Stability: expiration is 12 months from manufacturing date, frozen at -20 ℃ in PBS.

Applications

• As a research reagent (glycosaminoglycan degradation).
• For the preparation of disaccharides of heparan sulfate and the preparation of oligosaccharide libraries.

References

• McLean, M.W. Proc. 9th Int. Symp. Glycoconjugates, B-41, (1987)
• Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992)
• Pojasek, K., et al., Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III. Biochemistry 39, 4012-4019, (2000)

Order Information

Catalog number: AG00-8891

Packing size: 1 IU

Price: 2800USD