Heparinase II cleaves heparin and heparan sulfate. It is mainly used for quality control of heparin products.

General Information

Synonyms： Heparitinase II

Gene Source: Native flavobacterium heparinum

EC Number: Not assigned

CAS Number: 149371-12-0

MDL Number: MFCD00131319

Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.

Substrate Specificity: Heparin; heparan sulfate.

Properties

Molecular weight: 84.1 kDa

 

Isoelectric point: 9.1 – 9.2

 

pH optimum for activity: 7.6

 

pH range for activity: 5 – 9

 

Optimal temperature range: 20 ℃ – 37 ℃

 

Purity: ≥ 98 % by reversed phase HPLC analysis.

 

Specific Activity: 100 IU/mg, 1 IU/250 μL. One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30 ℃ and pH 7.5.

 

Stability: expiration is 12 months from manufacturing date, frozen at -20 ℃ in PBS.

 

Applications

• As research reagent (glycosaminoglycan degradation).
• For the preparation of di- and oligo-saccharides of heparin and heparan sulfate and the preparation of oligosaccharide libraries.

References

• McLean, M.W., et al. Proc. 8th Int. Symp. Glycoconjugates 1, 73, (1985)
• Moffat, C.F., et al. Proc. 8th Int. Symp. Glycoconjugates 1, 79, (1985)
• Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992)

Order Information

Catalog number: AG00-6512

Packing size: 1 IU

Price: 2300USD