Purpose
　　　This kit allows for the determination of EGR1 concentrations in Sheep serum, blood plasma, and other biological fluids.
Principle of the assay
　The kit assay Sheep EGR1 level in the sample，use Purified Sheep EGR1 antibody to coat microtiter plate wells, make solid-phase antibody, then add EGR1 to wells, Combined EGR1 which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EGR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.